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Recent advances in the fabrication of microfluidic platforms initiated during the late 90s have facilitated the realisation of micro total analysis systems. The integration of miniaturised fluidic handling and delivery systems with chemical and biochemical sensors provide applied scientists with powerful tools for in-field measurements away from central laboratories. Amongst the various classes of...
A 10 nm nanogold particle was used to label monoclone CEA antibody to prepare an immunonanogold resonance scattering probe (AuCEAAb) for detection of CEA. In the pH 6.8 Na2HPO4-NaH2PO4 buffer solution and in the presence of PEG-6000, the immunoreaction between CEA and AuCEAAb took place to form the immunonanogold complex that can be removed by centrifugation technique. The supernatant containing AuCEAAb...
Biosensors have a need to accurately detect and quantify a biological target within a complex medium, such as serum. Cells are uniquely qualified to accomplish this, but cannot communicate with the outside world. We demonstrate a synergistic fusion between a genetically engineered cell line and a wireless-compatible CMOS sensor leveraging the cells innate ability to specifically bind, amplify and...
An original protocol for the multicenter coordinated evaluation of seven analyzers for the measurement of ionized calcium is reported. The comparison of the instruments was made possible by using specific biological samples and newly developed HEPES or MOPS buffered aqueous reference solutions. The results for the evaluation of Ca/sup 2+/ instruments have shown that: (1) good precision and linearity...
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