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Raman spectroscopy is a rapid, non-invasive measurement mode that is readily able to discern bacteria from different genera (e.g. E. coli vs. Listeria spp.). At the species level, however, the task is more difficult. Herein we present a method of processing surface-enhanced Raman scattering (SERS) spectra from a low-cost, portable Raman system to discriminate between Listeria species. For six species...
Nanotechnology based detection of threat agents, such as explosives and biological agents, has been a top research priority at the Center for Chemical Sensors Development at the Department of Chemistry of the University of Puerto Rico-Mayaguez (UPRM). Nanoparticles are of fundamental interest since they possess unique size-dependent properties are quite different from the bulk state. When a bulk metal...
An unique, sensitive, and highly specific immunoassay system using gold nanoparticles and surface-enhanced Raman spectroscopy (SERS) is described. To demonstrate the analytical capabilities of the new technique, Raman reporter (mercaptobenzoic, MBA) that is coimmobilized with biospeciflc anti-protein A on gold nanoparticles (AuNP). Anti-protein A-AuNP-MBA, which combing both electromagnetic field...
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