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A cDNA encoding the myostatin (MSTN) was isolated from the Hubei white swine Longissimus dorsi muscle by RT-PCR, and corroborated through restriction enzymes map and sequencing. Identity analysis and, primary structure of translation alignment were made using NCBI-blast. Results revealed that the total length of the cloned MSTN gene was 1277 bp, and the opening reading frame (ORF) of MSTN was 1128...
Polyketide synthases are multifunctional enzymes that involve the synthesis of pigments and mycotoxin which is normally known as citrinin. The gene involving citrinin biosynthesis especially, acyltransferase in polyketide synthase gene of M. purpureus TISTR 3541 isolated in Thailand as the wild type and the ultrasonic induced mutation of this wild type as the mutants (generations 1-4, G1-G4) was investigated...
The purpose of this article was to observe interaction between drug and cancer cell with fluorescence probes and molecular imaging technology. In order to study the effect of cell growth and apoptosis morphology of Human gastric adenocarcinoma SGC-7901 induced by n-butanol extract from Capparis spionosa L. (CSBE), laser scanning microscopy was employed to observe apoptosis imaging. In addition, the...
In the ABC model, MADs-box proteins play an important role in floral development. As one of the MADs-box proteins, PI behaves as B function organ-identity genes acting in the second and third whorls of the flower to control petal and stamen identity. To find genes involved in the floral transition of pineapple guava, we cloned the full length cDNA encoding a MADs-box protein in Coolidge pineapple...
Endoplasmic reticulum stress(ERS)is a kind of sub-cellular pathological process which result from calcium disorderliness and protein improper folding. And endoplasmic reticulum stress induced apoptosis is a novel pathway which different from the mitochondria-initiated intrinsic pathway and the death receptor-triggered extrinsic pathway. Trichosanthin (TCS), a type I ribosome-inactivating protein,...
Objective: To construct the recombinant eukaryotic expression vector pcDNA3.1-CEMP1 containing the Human Cementum Protein 1 (hrCEMP1) by PCR and T/A cloning and establish NIH3T3 fibroblast cellline that stably expressing it. Methods: A pair of primers specific for amplifying the DNA fragment encoding hrCEMP1 were designed and synthesized. hrCEMP1 was inserted to the proper sites of vector pcDNA3.1...
In this study, differentially expression genes were isolated from a indica rice cultivar Zhong156 with a race-specific blast resistance gene subjected to the avirulent race ZC15 and the virulent race ZB1 of Magnaporthe grisea at the seedling growth stage. Twenty-five cDNA clones were isolated by using suppression subtractive hybridization combined with reverse Northern blot and confirmed by RT-PCR...
Tetherin cDNA was amplified from total RNA prepared from HeLa cells by RT-PCR, then cloned into the eukaryotic expression vector pEGFP-C3. The generated pEGFP-Tetherin construct was transfected into COS-1 to determine the expression and the subcellular localization of GFP-Tetherin fusion protein. Under CLSM the localization of GFP-Tetherin fusion protein distributed outside the nucleus and co-localized...
Studies about the variation of DNA methylation pattern under high temperature stress (37°C) contribute to a comprehensive understanding of the apparent regulation of biological functions. Based on the in vitro cultivation of genetically modified birch (Betula platyphylla Suk.) clones for high-temperature stress, the paper detected exogenous gene transcription level differences with RT-PCR. Methylation-sensitive...
CYP17 (17α-hydroxylase/17, 20-lyase) has been viewed as a critical enzyme for the biosynthesis of gonadal and adrenal steroids in vertebrates. According to a reported CYP17 gene of Strongylocentrotus purpuratus gene from NCBI, three pairs of primers were designed to clone CYP17 gene of Strongylocentrotus nudus,using RT-PCR. Three fragments were assembled into on contig of 1569bp, including full CDS...
Human Coagulation Factor IX (hFIX) plays a crucial role in the process of human endogenous blood clotting. Patients with deficiency of hFIX will suffer from hemophilia B. In this study, we use Pichia Pastoris to produce recombinant hFIX to cure hemophilia B Human liver hFIX cDNA were amplified by RT-PCR and inserted into pPIC9k vector, to construct pPIC9k-hFIX. The pPIC9k-hFIX were integrated into...
cDNA containing the 3' part sequences of seven Lily symptomless virus (LSV) isolates were obtained by RT-PCR and sequenced. The sequenced region is corresponding to partial CP gene and the entire sequences of 16kDa gene located at the 3' end of LSV genome. The sequence homologies of partial CP and 16 kDa protein genes, compared with other reported isolates, were 87.4% to 100% and 97.9% to 100% respectively...
SET and MYND domain-containing protein 3 (SMYD3) is a novel histone methyltransferase involved in human carcinogenesis. To establish the transgenic cell model of SMYD3 for the further studies on the characteristics and functions of SMYD3, in the present study, the human SMYD3 gene was subcloned into pIRES2-EGFP eukaryotic expressing vector. The recombinant plasmid was transfected into NIH3T3 cells...
Iron oxide nanoparticles(Fe3O4 NPs) have recently received increasing interest in the biotechnology and life science. However, little is known about the nanoneurotoxicity of Fe3O4 NPs following exposure to neurons. This study was to elucidate the cytotoxicity and its mechanism of Fe3O4 NPs on PC12 cells line which derived from Rattus norvegicus pheochromocytoma. The cell viability was observed by...
This study was comparison of MGMT and ERBB2 expression in human gliomas. RT-PCR method was used to determine the mRNA levels of MGMT and ErbB2 gene in 42 clinical glioma specimens,There was significant concordance between the expression of MGMT and ErbB2 (p < 0.01). MGMT and ErbB are highly remarkably related each other. The results suggest that there may be in relationship among MGMT and ErbB2...
To investigate the effect of shRNA-SR-PSOX (Scavenger receptor binds phosphatidylserine and oxidized lipoprotein) on smooth muscle cells (SMC) migration, we designed and synthesised the shRNA oligonucleotide targeted human SR-PSOX gene, and constructed the shRNA lentiviral expression system in vitro to infect HepG2 cell line, and the silencing efficiency was assayed by real-time reverse transcription-polymerase...
The satellite RNA of Bamboo mosaic virus (BaMV) contains an open reading frame for a 20-kDa nonstructural protein, which facilitates the long distance movement of satBaMV in plants. To determine whether the frame shift mutant of satBaMV (F6) could move systemically or could be complemented or promoted by P20, sucrose-synthasepromoter-driven P20 (SSP20) transgenic Nicotiana benthamiana plants have...
The wild boar OPN cDNA in size of 909bp was cloned by RT-PCR and analyzed by bioinformatics. The result showed that wild boar OPN contains 1) a potential calcium phosphate apatite binding region of eight consecutive Asp residues beginning at amino acid 86, 2) a cell-attachment Gly-Arg-Gly- Asp-Ser (GRGDS) sequence (residues 153-157), 3) a thrombin RS cleavage site (residues 163 and 164). The phylogenetic...
The partial sequence of Skeletonema costatum proliferating cell nuclear antigen (PCNA) gene was obtained by RT-PCR. A 714 bp cytochrome b (Cyt b) gene fragment of S.costatum was cloned for the first time. Based on the cloned sequences, a FQ-PCR method was developed to detect the S.costatum PCNA and Cyt b gene, respectively. The above method was applied to study the relationship between the S.costatum...
Evaluate the possible value of FasL in gastric cancer gene therapy by investigating the effects of FasL expression on human gastric cancer cell line.An adenoviral vector encoding the full-length human FasL cDNA was constructed and used to infect a human gastric cancer (SGC-7901) cell line. FasL expression was confirmed by X-gal staining, flow cytometric analysis and RT-PCR. The effect of FasL on cell...
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