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Ribosomal protein S18 is a component of the 40S subunit, encoded by the RPS18 gene in eucaryotic cell, belonging to the S13P family of ribosomal proteins. To explore the structure characteristic of RPS18 gene of the Ailuropoda melanoleuca, primers we re designed based on the known nucleotide sequence of RPS18 gene to clone the cDNA and genomic sequences of RPS18 gene of A. melanoleuca by RT-PCR and...
interferen-? of Rongchang porcine was amplifed by RT-PCR with the total RNA extracted from the peripheral blood lymphocytes. The gene was sequenced after cloned into the vector pMD18-T and analyzed with molecular biological software. The results showed that the opening reading frame (ORF) of interferen-? of Rongchang porcine consists of 501 nucleotides, encoding 166 amino acids, the front 23 amino...
A novel Or83b was cloned from antennae of Heliothis viriplaca (Lepidoptera: Noctuoidea) by using RT-PCR and RACE methods, which was named HvirOr83b (GenBank accession no. JQ394904). The cloning and sequencing results showed that the full-length of open reading frame in HvirOr83b was 1422 bp, encoding 473 amino acid residues with seven transmembrane domains, which shared typical feature of Or83b with...
A pair of degenerate primers was designed according to the conservative regions at the 3' terminus of the floral meristem identity genes (LEAFY). A 361 bp cDNA fragment of LEAFY homologous gene (PcBLFY) coding for 120 amino acid residues was amplified via RT-PCR from flower buds of male, female and hermaphrodite plant of Pistacia chinensis Bunge respectively. Furthermore, 989 bp genomic fragment of...
Amomum villosum Lour. (officinal name Fructus Amomi) is one of the most well-known and authentic herbs in South China. Monoterpenes in its volatile oil are the main medicinal compounds. The condensation of pyruvate with D-glyceraldhyde-3-phosphate to form 1-deoxy-D-xylulose-5-phosphate (DXP) catalyzed by DXP synthase (DXS, EC: 2.2.1.7) and the conversion of DXP to 2-C-methyl-D-erythritol-4-phosphate...
Soil salinity has become the most significant a biotic stress, resulting in inhibition of plant growth and even death. To search a novel molecular mechanism involved in salt stress, the translation initiation factors (eIFs) including eIF1, eIF1A, eIF4, and so on, have been found to have potentials to response salt stress. Increased expression has been found particularly in eIF1 and eIF1A, which both...
The cDNA of DREB transcription factor named OjDREB1 was obtained from Lilyturf (Ophiopogon japonicus) under the drought stress of 12h by RT-PCR method. Sequence analysis showed that cDNA of OjDREB1 is 1779 bp long, the open reading frame is 1062bp.The predicted protein has 353 amino acids with an estimated molecular mass of 38.69 kDa and an isoelectric point of 6.46. The deduced primary structure...
Cloning of new NAC transcription factors from maize (Zea mays). The length of new ZmNAC cDNAs were cloned by RT-PCR, designing the degenerate primer based on the conserved domains of NAC transcription factor. The length of ZmNAC cDNA sequence was isolated from maize, and it contain 750bp more or less. The results showed that a new center sequence of ZmNAC transcription factor was cloned in maize.
This project utilize surgery combine chemical method to establish rat model of thromboangitis obliterans. To appraise the model we use the method of pathology, immunology and molecular biology. The result shows that injection with laurate sodium to Femoral Artery can successfully establish rat model of thromboangitis obliterans that similar to human natural disease. Rat model pathological changes...
A full length dicer-like gene was cloned from 9311 (Oryza sativa indica) by RACE (GenBank accession No: DQ208406). It should be an OsDCL3b through homogeneous alignment and locate on chromosome 10. This gene might have alternative splicing transcripts. Real-time PCR results indicated that it expressed at different levels in root, stem, leaf and flower, respectively. The highest expression level of...
To better understand soybean ferritin gene structure and its expression, a full length of 753bp cDNA fragment, which encoded a 250 amino acids peptide, was cloned by RT-PCR from a cultivar of Zhong Huang 13. Multiple sequence alignment and phylogenetic analysis were performed with ferritins from 6 species including Glycine max, Arabidopsis thaliana, Oryza sativa, Physcomitrella patens, Brachypodium...
To better understand the osmo-adaption mechanism in the Torulopsis versatilis, we investigated the salt-tolerant gene HOG1 from the wild-type T. versatilis. Genome of T yeast was used for PCR reaction to amplify the HOG1 gene. The PCR productions were sent into sequencing vector pUC19 to achieve the plasmid pUC19-HOG1. The engineered strain W303-YEp195-T and the control strain W303-YEp195 were constructed,...
We isolated the coding sequence a putative SbCO gene from Sorghum cultivar, Roma, by in silico sequence analysis and subsequently, reverse-transcription PCR. The DNA sequencing data revealed 92% similarity between SbCO and the Arabidopsis flowering time regulator CONSTANS. To investigate the biological function of SbCO in sorghum, we have constructed an inducible expression vector for SbCO based on...
This study was aimed to isolate ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit (rbcL) from lilyturf (Ophiopogon japonicus). A pair of primers used in amplifying chloroplast fragment from lilyturf was designed according to the corresponding sequences in tobacco, spinach chloroplast genomes. The fragment containing rbcL gene was cloned from lilyturf chloroplast genome. Sequencing analysis...
Thromboangitis obliterans is a kind of common peripheral vascular disease, takes an important part in peripheral vascular disease. The cause of thromboangitis obliterans is complex and uncertain, was presumed as the result of synthetic action of several factors. The therapeutic effect of Qing sui yu ju dan to thromboangitis obliterans is certain, while the curing mechanism is indefinite. In the experiment,...
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