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A Carica papaya pectin methylesterase (CpL-PME) present in a commercial papain preparation was used to modify the amount and distribution of anionic charge in a population of a model pectic homogalacturonan (HG) at pH 4.5 and pH 7.5. Introduced negatively charged demethylesterified blocks (DMB) were excised as oligomers with limited endo-polygalacturonase (EPG) digestion, and then separated and quantified...
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