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Pearl gentian grouper, Epinephelus lanceolatus × Epinephelus fuscoguttatus, is a hybrid species with increasing market requirement in China. A disease outbreak occurred in the fish juvenile stages in Shandong Province, P.R. China. The gross signs were characterized by mass mortality, exhibiting dark skin coloration, reduce of feeding and having abnormal swimming behavior. Based on histopathological...
40S ribosomal protein S10 is a protein encoded by the RPS10 gene in human, belonging to the S10E family of ribosomal proteins. To explore the structure characteristic of this gene of Ailuropoda melanoleuca, primers were designed based on the known nucleotide sequence of RPS10 gene. The RT-PCR and PCR strategy were employed to isolate cDNA and genome of RPS10 from A. melanoleuca respectively, followed...
cDNA containing the 3' part sequences of seven Lily symptomless virus (LSV) isolates were obtained by RT-PCR and sequenced. The sequenced region is corresponding to partial CP gene and the entire sequences of 16kDa gene located at the 3' end of LSV genome. The sequence homologies of partial CP and 16 kDa protein genes, compared with other reported isolates, were 87.4% to 100% and 97.9% to 100% respectively...
Pear is a commercially important fruit tree widely distributed in China that displays GSI (gametophytic self-incompatibility). It is necessary to determine S-alleles and S-genotypes for clarifying the compatibility between cultivars prior to pear planting and breeding. The S-genotype analysis using conventional method of artificial pollination is time consuming and requires much labor. In addition,...
RPL18A is a component of the 60S large ribosomal subunit encoded by RPL18A gene, which plays an important role in ribosome. To explore the structure characteristic of ribosomal protein L18 (RPL18A) gene of the Giant Panda (Ailuropoda melanoleuca) and the differences with other species reported,we have designed primers which based on the already-known information of RPL18A gene. Then, the cDNA and...
The wild boar OPN cDNA in size of 909bp was cloned by RT-PCR and analyzed by bioinformatics. The result showed that wild boar OPN contains 1) a potential calcium phosphate apatite binding region of eight consecutive Asp residues beginning at amino acid 86, 2) a cell-attachment Gly-Arg-Gly- Asp-Ser (GRGDS) sequence (residues 153-157), 3) a thrombin RS cleavage site (residues 163 and 164). The phylogenetic...
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