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Prostate cancer (PCa) is the second most common type of cancer in the world and the fifth highest cause of cancer-related deaths in men [1], with the highest prevalence in the United States and Western Europe. The current diagnostic gold standards are controversial and typically lead to over-diagnosis [2]. Blood tests are commonly used to check the level of the prostate specific antigen (PSA), when...
A droplet based microfluidic system encapsulating PCR reagent into alginate beads as a new digital PCR solution has been developed, which avoided droplet coalescence and enabled efficient DNA amplification and high-throughput fluorescent imaging. We also compared two trapping methods using hydrodynamic traps and micro-sieves, and indicated the advantages of latter for higher trapping rate and no droplet...
Two-photon absorption fluorescence imaging is used to quantitatively measure 3D flow and mixing in microfluidics. This is an important characterization tool for developing optimal microfluidic devices for use in the study of biological molecular dynamics.
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