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Concerns over the use of steroid hormones as growth promoters, have prompted the EU to prohibit their use in food producing animals. Subsequently, rigorous screening procedures have been implemented in all member states to detect the illegal administration of such compounds. In the presented work, disposable immunosensors for the detection of 19-nortestosterone and methyltestosterone in bovine urine...
This work reports on the development of rapid enzyme-linked immunosorbent assay for testosterone, methyltestosterone, 19-nortestosterone and their metabolites in real samples of bovine urine. The assays were based on the competition between an immobilised testosterone–BSA conjugate and the analyte for corresponding antibodies, followed by the use of secondary anti-species (α-IgG-HRP) to determine...
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