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The pressure inside large arteries is typically ~100 mmHg higher than outside of them. This difference, ??P, drives a flow of plasma, mainly water and advected/swept-along solutes, that would otherwise only transport by (much slower) diffusion [1], across the porous vessel wall. It is natural to ask whether this flow enters the wall across the endothelium solely through inter-endothelial cell (EC)...
Potassium channels are membrane proteins that allow the passage of potassium ions at near diffusion rates while severely limiting the flux of the slightly smaller sodium ions. Based on the three dimensional structure of KcsA with a D- alanine at position 77 (KcsAD-Ala77), the authors use a hierarchical model of K channel selectivity permeability process involving ab initio quantum calculations and...
We present a highly parallel and reproducible method for reconstituting an array of lipid bilayers to analyze membrane transport. We infuse buffer/lipid/buffer solutions sequentially into a microchannel with numerous microchambers in its walls and seal each chamber by a lipid bilayer containing membrane proteins. Due to the small volume of the chamber (2 pL), membrane transport of confined fluorescent...
A group of membrane proteins endowed with a single C-terminal hydrophobic domain capable of insertion into lipid bilayer is known as tail-anchored (TA) proteins. We analyzed the integration of the TA domain of holo-form of human cytochrome b5 (HCYTb5) into protein-free liposomes different in a lipid composition. The integration of holo-b5 occurred efficiently into membranes with low cholesterol content...
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