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Application of wastewater to soil may result in the contamination of groundwater and soil with pathogenic microorganisms and other biological and chemical agents. This study was performed to determine the antiviral microbial activity of soil saturated with secondary effluent. Low concentrations (0.05mg/ml) of protease pronase resulted in the inactivation of more than 90% of seeded Cox-A9 virus, whereas...
Nucleic acid (NA) amplification techniques are useful to detect viruses in water and other environmental samples because they are highly sensitive, specific and can detect fastidious enteric viruses that do not grow well or not at all in cell cultures. However, RT-PCR was found to detect inactivated viruses. In terms of risks to public health this constitutes a false positive result, as inactivated...
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