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The growth/no growth responses of Listeria monocytogenes inoculated at four levels (0.90, 2.58, 4.20 and 6.81 log cfu/well; 300 μl medium/well) into tryptic soy broth (TSB) were monitored at different combinations of temperature (4 to 30 °C), pH (3.76 to 6.44) and a w (0.888 to 0.997) for 60 days. The study was conducted in 96-well microtiter plates and growth was monitored visually and by...
The growth/no growth response of a mixture of five strains of Listeria monocytogenes in tryptic soy broth and on tryptic soy agar was monitored in combination treatments of temperature (4-30 o C), pH (4.24-6.58) and a w (0.900-0.993) for 30 days. A significant difference between the growth limits of the pathogen in broth and agar was observed. At 25 o C the minimum pH values...
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