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Automatic microscopy for screening of sputum smears for tuberculosis would reduce the reliance on technicians in heavily burdened laboratories in poorly-resourced countries. Autofocusing is a key component of automated microscopy. We investigate the use of wavelet-based image fusion for automatic focusing of sputum smear slides as a component of automated fluorescence microscopy to identify Mycobacterium...
In this paper, we consider the problem of the accuracy of estimating the location and other attributes of a moving single molecule whose trajectory is imaged by fluorescence microscopy. As accuracy in parameter estimation is closely related to the Fisher information matrix, we first give a general expression of the Fisher information matrix for the estimated parameters for a single object moving in...
We report a highly sensitive imaging technology using surface-enhanced Raman scattering nanoparticles. Fluorescence microscopy is a well-known imaging technique which provides a specific protein distribution inside cells. However, most of currently available fluorescent organic dyes have relatively weak emission intensities and they are also photo-bleached quickly. In order to resolve this problem,...
We have utilized basic principles of optical interference and resonance and applied our experience in resonant optoelectronic devices to biological applications demonstrating nanometer scale measurement capability in fluorescence microscopy and label-free sensing of protein binding in a high-throughput micro-array format. A novel application of resonance to fluorescence microscopy promises nanometer...
The influences of host-guest interactions on the emission properties of enhanced green fluorescent protein (EGFP) embedded in mesoporous silica particles are studied in detail by fluorescence spectroscopy and microscopy. Silica particles from the MCM-41, ASNCs and SBA-15 families that all possess nanometer-sized (3-8 nm in diameter) channels but different particle geometry and pore sizes (distribution)...
This paper presents the design, fabrication, and the characterization of a photo-response compensated piezoresistive cantilever. The application field of the cantilever is to measure cellular mechanical properties under fluorescence microscopy. Photo-response which is caused by an excitation light of a fluorescence microscope interferes with measurement. We proposed piezoresistive cantilevers consist...
In an effort to manufacture a high-performance disposable microfluidic system for applications in medical diagnostics, a series of studies were carried. Surface modification on COC, nanoparticle-based immunoassays on COC, manufacturing cross flow microfluidic system, synthesis of a pair of molecules for fluorescence resonance energy transfer (FRET) and preparation of a FRET nanoparticle are presented...
Fluorescence microscopy allows the acquisition of the spectroscopic properties of fluorescent reporter molecules at levels of resolution too small to be seen with the naked eye. The Indirect Immune Fluorescence Test is the method used to identify antinuclear antibodies. The main principle of this method is to identify the auto-antibodies in a patient's blood serum by staining affected cell structures...
Membrane dynamics plays an essential role for cellular processes. Here we demonstrate the application of several biophotonic techniques, such as advanced fluorescence microscopy and laser spectroscopy, to study some fundamental membrane biology problems.
By combining optical manipulation, fluorescence microscopy and microfluidic devices we have studied the response from different signaling pathways in single Saccharomyces cerevisiae cells upon environmental perturbations. GFP-tagged proteins in the signaling pathway of interest visualized the heterogenic response within a population.
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