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We developed selective cell injection of nanotool using photo responsive chemical for intracellular measurement. The nanotool was included in the fusogenic liposome. Membrane fusion of the liposome to the cell membrane was used for invasive cell injection of the nanotool. The liposome fuses to the cell in weak acidic condition. Local pH control inside the liposome was developed by using photo responsive...
We present a continuous glucose monitoring sensors using glucose-sensitive fluorescent dyes. We prepared microgels by copolymerizing acrylamide with the glucose sensitive fluorescent dyes. This fluorescent gel shows a high quantifiability and rapid response in the physiological glucose concentration range of 62.5 to 1000 mg/dL. Our in vivo study revealed that fluorescent intensity of the gels changed...
Voltage-sensitive dyes (VSDpsilas) undergo changes in their electronic structure, and consequently their fluorescence spectra, in response to changes in the surrounding electric field and are therefore used for recording of monophasic action potentials (MAPs) by optical method. S.-c. phototoxic effects of VSDs appear under intense illumination and include alterations in heart electrical signals. To...
Action potential alternans has been an indicator of ischemic disease and vulnerability to ventricular fibrillation (VF). The mechanisms of alternans are linked to the anomalies in intracellular Ca 2+ (Cai) handling by either spontaneous Ca2+ release or modulation of action potential duration (APD), which may promote wave breaks in VF. We investigated possible role of Ca2+ in wave breaks by simultaneously...
Fast processes in cardiac electrophysiology are often studied at temperatures lower than physiological. Extrapolation of values is based on widely accepted Q10 (Arrhenius) model of temperature dependence (ratio of kinetic properties for a 10degC change in temperature). In this study, we set out to quantify the temperature dependence of essential parameters that define spatiotemporal behavior of cardiac...
Action potential alternans has been an indicator of ischemic disease and vulnerability to ventricular fibrillation (VF). The mechanisms of alternans are linked to the anomalies in intracellular Ca 2+ (Cai) handling by either spontaneous Ca2+ release or modulation of action potential duration (APD), which may promote wave breaks in VF. We investigated possible role of Ca2+ in wave breaks by simultaneously...
Fast processes in cardiac electrophysiology are often studied at temperatures lower than physiological. Extrapolation of values is based on widely accepted Q10 (Arrhenius) model of temperature dependence (ratio of kinetic properties for a 10degC change in temperature). In this study, we set out to quantify the temperature dependence of essential parameters that define spatiotemporal behavior of cardiac...
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