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To quantify the permeability of rat pial microvessels, we measured the apparent microvessel permeability (P) to various sized solutes. Rats (SD, 250-300 g) were anesthetized with sodium pentobarbital given subcutaneously and kept warm on a heating pad. The pial microcirculation was observed by a high numerical aperture objective lens through a section of frontoparietal bones thinned with a high-speed...
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