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Both x-ray diffraction and x-ray fluorescence techniques have proven beneficial for identifying and characterizing biological tissues. X-ray diffraction analysis uses the elemental composition of the sample to discriminate between tissue types, and can provide a considerable enhancement in contrast over conventional x-ray imaging. X-ray fluorescence, in this case, is being used to locate the presence...
Panoramic images providing a larger local overview of the internal urinary bladder wall can be used for documentation and surgery planning, as well as assist the re-identification of multi-focal tumors during a cystoscopy. In contrast to white light illumination, the photodynamic diagnosis (PDD) leads to an enhanced tissue contrast, resulting in a reddish fluorescence of malignant tissue excited by...
We have developed a dual-modality imaging system by integrating optical-resolution photoacoustic microscopy and fluorescence confocal microscopy to provide optical absorption and fluorescence contrasts simultaneously. By sharing the same laser source and objective lens, intrinsically registered photoacoustic and fluorescence images are acquired in a single scan. The micrometer resolution allows imaging...
We report the development and validate a multi-modal tissue diagnostic technology, which combines three complementary techniques into one system including ultrasound backscatter microscopy (UBM), photoacoustic imaging (PAI), and time-resolved laser-induced fluorescence spectroscopy (TR-LIFS). UBM enables the reconstruction of the tissue microanatomy. PAI maps the optical absorption heterogeneity of...
We demonstrate a novel microelectromechanical system (MEMS) based Dual-Axes confocal (DAC) microscope in a 10-mm diameter handheld package. Miniaturization is achieved by using a barbell-shaped, gimbaled, two-dimensional (2-D) MEMS scanner that is actuated by self-aligned vertical comb actuators. The maximum DC optical scan angles are plusmn4.25deg on the inner axis and plusmn1.5deg on the outer axis,...
Because of the overwhelming scattering of light in biological tissues, the spatial resolution and imaging depth of conventional fluorescent imaging is unsatisfactory. Therefore, we present a dual modality imaging technique by combining fluorescence imaging with high-resolution noninvasive photoacoustic tomography (PAT) for the study of an animal tumor model. PAT provides high-resolution structural...
Although detection of tumor cells in peripheral blood using imitiunocytochemistry and optical scanning is a promising method for screening and monitoring cancer, it poses a major technical challenge due to the extremely low tumor cell concentration in blood. The preferred detection method - digital microscopy - is far too slow for analysis of the large numbers of cells required for statistical validity...
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