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We applied differential scanning calorimetry (DSC) to investigate the effects of substitutions D137L and G126R (i.e. replacement of conserved non-canonical residues Asp137 and Gly126 by canonical ones) in the middle part of tropomyosin (Tm), as well as the combined one, D137L/G126R, on the thermal unfolding of Tm. Special approaches (e.g. combination of DSC with measurements of temperature dependences...
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