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Galium verum L. is a wild plant from Northeast China, this grass is well known as an excellent food source of vitamin, and it also contains phytochemicals such as chlorogenic acid,salicylic acid and flavone. The present work evaluates the anti-venous thrombosis capacity of hydrophilic extracts of Galium. Anti-venous thrombosis activity values obtained for Galium were higher than those reported for...
In order to analysis of chlorogenic acid in Do-Zhong(Eucommia ulmoides), ultrasolic extraction was used to prepare the sample. With yield of chlorogenic acid as index. The effect of ethanol concentration, ultrasolic power, extraction time, and the ratio of solid to liquid were studied. The extracts were then analysis by high-performance liquid chromatographic (HPLC) method. The results shown that...
Various extracts of Wikstroemia chamaedaphne were evaluated their acaricidal activities against Tetranychus cinnabarinus (Boisduval) and Tetranychus urticae Koch (Acari: Tetranychidae) in laboratory. According to our study, the Wikstroemia chamaedaphne extracts had significant acaricidal activities against T. cinnabarinus and T. urticae. In this study, the mean LC50 value for T. cinnabarinus and T...
Few studies mentioned acaricidal activity of Wikstroemia chamedaphne against Tetranychus viennensis. Here, we reported that W. chamedaphne had strongly acaricidal activity against T. viennensis. A method for purification of miticidal components from W. chamedaphne was developed as follows: Step 1, methanol immersion at room temperature; Step 2, liquid-liquid partition using petroleum ether; Step 3,...
Most plants could produce bioactive substances including alkaloids, flavonoids, terpenes and relative materials, which are poisonous or have antifeedant effect to herbivorous animals, including some insects. Some well-known pesticides originated from plant bioactive substances have been successfully identified and used, for example, the azadirachtin from neem, nicotine from tobacco, rotenone and deguelin...
The chemical composition of fresh Cinnamomum camphora were separated and analyzed by GC/MS. It was determined that 17 relative content by area normalization. 17 compounds of the extractives were identified. The main and abundant constituents were Eucalyptol, 3-Cyclohexen-1-ol,bicyclo[3.1.0]hexane, 4-methylene, beta.-Pinene, 3-Cyclohexen-1-ol, 4-methyl-1-(1-methylethyl), 1,3-Cyclohexadiene, 1-methyl-4-(1-methylethyl),...
Abundant leaves and kernel hulls of Tea-oil tree (Camellia oleifera) are still underutilized and wastefully abandoned to pollute environment. In order to solve this problem and better utilize the leaves and kernel hulls of C. oleifera, we used GC/MS technology to analyze the top value-added bioactive constituents of their benzene/ethanol extractives. After treated with freeze-drying, the fresh leaves...
In this paper, Southern blot analysis of the transgenic birch (Betula platyphylla Suk.) plants indicated that the the copy number of bgt varies from one to four. Of which 18.2 percent were single copy. Transcriptional expression of bgt gene in transgenic birch plants was analyzed by Northern blot. TGS has been found in four transgenic lines with one, three and four copies of bgt. Bgt gene was inserted...
As the most expensive wood tree, Santalum album has been planted more than 2000 mu in South China now. A mass of Santalum album leaves was naturally abandoned and degradable so that the resources were wasted. Therefore, in order to overcome the negative effects, Py-GC/MS was used to analyze the extractives of autumn Santalum album leaves. Relative content of each component was determined by area normalization...
Objective: To find the antitumor compounds from Rabdosia Serra (Maxim) Hara, phytochemical isolation procedures were performed by bioassay-guided fractionation. Methods: The ethanol extract of Rabdosia serra (Maxim) Hara was fractionated by organic solvents-petroleum ether, benzene, ethyl acetate and butanol in sequence. The in vitro antitumor activity against human hepatoma cell line HepG2 and human...
The head-space (HS) volatile compounds of the buds and flowers of Prunus persica (L.) Batsch. f. duplex Rehd were analysed by solid-phase microextraction (SPME) coupled with capillary gas chromatography/mass spectrometry (GC-MS). Typical compounds detected in P. persica (L.) Batsch. f. duplex Rehd buds and flowers were alkanes. Tetradecane (4.07%), 4,8- dimethyl undecane (4.56%), pentadecane (11.3%)...
The chemical composition of six gymnosperm species, Sabina chinensis L. Art, Cedrus deodara Roxb G. Dor, S. chinensis (L.) Art, P. bungeana Zuccex Enall, Platgcladus orientalis Franco and Torryeya grandis Fortcv.Merrillii, and volatile oils obtained from the leaves by steam distillation was analysed using gas chromatograph and mass spectroscopy analysis (GC-MS). To evaluate in vitro antimicrobial...
In order to better utilize and recover the productive wastewater from the Eucalyptus oil and hence decrease its pollution to water environment, we used GC/MS to analyze the possible top value-added components of benzene/ethanol extractives of leaves of two Eucalyptus cultivars, i.e. E. camaldulensis and E. citriodora . Relative content of each component was determined by area normalization. 22 compounds...
Roots were obtained from aseptic seedlings and used as materials for cultures on agar medium and liquid medium, and the production of comptothecin (CPT) in the roots was analyzed with histochemical means and thin layer chromatography. Results of the experiments showed that in vitro cultured roots could not only produce CPT, the ability of production was even higher than seedling roots. Moreover, it...
The chemical components from benzene/ethanol extractives of fresh Castanopsis fissa wood were separated and identified by GC/MS after enriched. Relative content of each component was determined by area normalization. 18 compounds representing 83.52% of the extractives were identified. The main and abundant constituents were 2-furancarboxaldehyde, 5-(hydroxymethyl)-(22.45%), D- mannose(19.61%), vitamin...
The extractives of Eucalyptus urophydis wood consumes more bleaching agents which seriously affect the quality of pulp and paper, and increases more difficulties on the treatments of wastewater and black-liquid from wood industry, especially pulp- and paper-making industry. Therefore, In order to solve the problem, Py-GC/MS was used to analyze the high- grade resource recovery of benzene/ethanol extractives...
The fruit hull and kernel hull of Camellia oleifera are still underutilized and hence abandoned to pollute environment. Therefore, Pyrolysis- GC/MS was used to analyze the top value- added biomedical components from the fruit hull and kernel hull of C. oleifera fruit. After treated with freeze-drying, the fresh fruit hull and kernel hull were pyrolyzed in Helium atmosphere at 590degC respectively,...
The extractives from masson pine (Pinus massoniana L.) bark has been used in medicine successfully. Therefore, Py-GC/MS was used to analyze the high-grade resource recovery of benzene/ethanol extractives of masson pine wood. Relative content of each component was determined by area normalization. The main and abundant constituents of 590deg pyrolysis product of masson pine wood werel-phenanthrene...
As the noble gardening tree growing specifically in China, Osmanthus fragrans is widely used to produce high-grade essence, special drugs and aromatic tea form its flowers. However, O. fragrans leaves are still underutilized and hence wasted. Therefore, in order to better utilize O. fragrans leaves and hence to expand its utility values and industrial scopes, we attempted to analyze the bioactive...
As the most expensive wood tree, Santalum album L. has been planted more than 1000 mu in South China now. A mass of S. album L. shoots was naturally abandoned and degradable so that the resources were wasted. Therefore, in order to overcome the negative effects, Py-GC/MS was used to analyze the extractives of S. album L. shoots. Relative content of each component was determined by area normalization...
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