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In the present study, bacterial magnetosomes were used as a carrier molecule to couple with lemon grass extract (LGE) for the prevention of microbial biofilm in wound dressing material. Magnetosomes were extracted from Magnetospirillum sp. VITRJS-1 and characterised by microscopic and X-ray diffraction analysis. The phytochemical analyses of the extract showed the presence of bioactive compounds that...
In the present study, an efficient biosurfactant producing bacterial strain Pseudomonas aeruginosa MKVIT3 was isolated from an oil logging area in Vellore district of Tamil Nadu, India. Liquid chromatography–mass spectrometry (LC-MS/MS) analysis was performed for the identification of different congeners present in the extracted biosurfactant. The column purified biosurfactant was used to stabilise...
Non-mitogenic form of recombinant Human acidic fibroblast growth factor (nrhaFGF) retained the cardio and neuro-protective characters of the wild-type aFGF while decreased the potential side effects like mitogenesis activity. In this paper, nrhaFGF was successfully expressed in Escherichia coli and yielded about 5% of the total soluble protein in the 10L fermentation tank. the succeedent chromatography,...
The disinfection effect of Copepod of zooplankton with chlorine dioxide was compared with that of chlorine. The experimental results showed that chlorine dioxide was more effective to kill Copepod. Copepod was completely killed by 1.0mg/L of chlorine dioxide for contacting 30 min but the required dosage were about 2.0mg/L of liquid chlorine. The body structure variety of Copepod indicated that the...
Through indoor experiments, concentration changes of n-hexadecane in the cells of bacillus cereus DQ01 and bacillus sp.DQ02 with the time, inhibition effect of NaN3 on the transportation of n-hexadecane across the membrane, as well as the accumulated form of n-hexadecane in the cells of bacteria were studied. The results showed that the higher the content of n-hexadecane in the medium was, the more...
Mycelium is a kind of residue which is filtrated and extracted from the microbial fermentation process in the pharmaceutical industry. Some samples of mycelium were taken from North China Pharmaceutical Factory. In order to investigate their physical and chemical characteristics, with the gravimetric method, atomic absorption, K2SiF6 volumetric method, EDTA volumetric method, copper titration method...
Spectrally resolved imaging was applied to study the growth dynamics of phototrophic biofilms comprizing a mixture of one cyanobacterial and one diatom species. Linear spectral unmixing was combined with liquid chromatography to quantitatively discriminate the areal biomass densities of the two populations. The grown biofilms exhibited highly heterogeneous distribution with patches of 1-2 mm in size,...
Nucleotide sequences of the 16S rRNA gene of Thermobifida (formerly Thermomonospora) Q-0 was analyzed by MEGA v.3, and the phylogenetic tree showed the relationship between different strains in Thermobifida genus. A carboxymethyl cellulase I (CMCase I) was purified by fractional ammonium sulphate precipitation and ion exchange chromatography on DEAE-Sephadex. The enzyme showed the highest activity...
Hirudin has been well-characterized to be the strongest thrombin inhibitor with no affinity for other peptidases. Hirudin from natural sources is inefficient due to its low abundance in medicinal leech (Hirudo medicinalis). Though recombinant hirudin has been made by many laboratories, technical difficulties have limited recombinant hirudin from being used clinically. One of the difficulties is the...
Tumstatin anti-tumor peptide of 19peptide can inhibit the proliferation of melanoma tumor cell. To study its effect on the proliferation and apoptosis of different cells and verify its no anti-tumor specificity, the base sequence of 19peptide was designed and constructed engineering bacteria. The soluble 19peptide was obtained from one step chitin affinity chromatograph. By such experiment as MTT...
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