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To explore the inhibition of juglone in Qinglongyi on A-549 cells in vitro, MTT assay was used. Laser confocal scanning microscope was used to observe apoptotic morphology. Changes of cell cycle are studied by flow cytometry analysis. The result of MTT assay showed that juglone had a marked growth inhibition in A-549 cells and the IC50 is respectively 3.4 × 10-5 mol/L, 1.8 × 10-5 mol/L and 2.6 × 10...
Non-mitogenic form of recombinant Human acidic fibroblast growth factor (nrhaFGF) retained the cardio and neuro-protective characters of the wild-type aFGF while decreased the potential side effects like mitogenesis activity. In this paper, nrhaFGF was successfully expressed in Escherichia coli and yielded about 5% of the total soluble protein in the 10L fermentation tank. the succeedent chromatography,...
Bifunctional alkylating agent 1,3-Bis-(2-chloroethyl)-1-nitrosourea (BCNU) is a significant anticancer drug in the clinical treatment of human malignancies. Several lines of evidences indicated that the cytotoxic activity of BCNU was related to their ability of inducing DNA interstrand cross-links (ICLs). In the present work, the formation of the ICLs from the treatment of calf thymus DNA with BCNU...
To explore how to calculate the effect of solanine on the Michaelis constant and the maximum reaction rate of NAT, high performance liquid chromatography (HPLC) was used, with 2-AF as substrate, and the rate at which 2-AF is acetylated into 2-AAF in intact HepG2 cells or in the cytoplasm of HepG2 cells as the reaction rate. The double reciprocal plot was made, with 1/S (reciprocal of the concentration...
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