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In this work, N, O-carboxymethyl chitosan (CMCS) samples from virgin chitosan (CS) were synthesised and CMCS/polyethylene oxide (PEO) (50/50) blend nanofibrous samples were successfully electrospun from their aqueous solution. The electrospinning conditions to achieve smooth and fine diameter nanofibrous mats were optimised via D-optimal design approach. Afterwards, vitamin C and phenytoin sodium...
Mycelium is a kind of residue which is filtrated and extracted from the microbial fermentation process in the pharmaceutical industry. Some samples of mycelium were taken from North China Pharmaceutical Factory. In order to investigate their physical and chemical characteristics, with the gravimetric method, atomic absorption, K2SiF6 volumetric method, EDTA volumetric method, copper titration method...
In the present study, a method is described for the determination of ATP in small samples of interstitial fluid (< 100 mul) using microdialysis combined with ion-pairing high performance liquid chromatography (HPLC) in a gradient elution system which gives a clear separation of ATP from other interstitial constituents. Lactic acid infusion was performed to stimulate ATP to exit the cells to appear...
To explore how to calculate the effect of solanine on the Michaelis constant and the maximum reaction rate of NAT, high performance liquid chromatography (HPLC) was used, with 2-AF as substrate, and the rate at which 2-AF is acetylated into 2-AAF in intact HepG2 cells or in the cytoplasm of HepG2 cells as the reaction rate. The double reciprocal plot was made, with 1/S (reciprocal of the concentration...
To explore how to calculate the effect of solanine on the Michaelis constant and the maximum reaction rate of NAT, high performance liquid chromatography (HPLC) was used, with 2-AF as substrate, and the rate at which 2-AF is acetylated into 2-AAF in intact HepG2 cells or in the cytoplasm of HepG2 cells as the reaction rate. The double reciprocal plot was made, with 1/S (reciprocal of the concentration...
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