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We investigated the relationship between plant growth and polyphosphate (poly P) content in Allium fistulosum roots colonized by Glomus sp. R10 or Glomus etunicatum with different levels of phosphate addition. Poly P was quantified by two enzymatic assays: long-chain poly P (>∼20 inorganic phosphate [P i ] residues) with polyphosphate kinase, and total poly P with polyphosphate exopolyphosphatase...
Polyphosphate is ubiquitous among living organisms and has a variety of biochemical functions. Arbuscular mycorrhizal fungi have been known to accumulate polyphosphate as a key compound for their function. However, an enzymatic assay using polyphosphate kinase (PPK) reverse reaction, in which polyphosphate is converted to adenosine triphosphate (ATP) and quantified by luciferase assay, failed to detect...
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