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The dicarbonyl compounds glyoxal, methylglyoxal, and dimethylglyoxal have been separated by capillary GC on a 30 m × 0.32 mm i.d. HP-5 column after precolumn derivatization with 2,3-diamino-2,3-dimethylbutane at pH 4. Chromatographic separation was complete in 6 min. Nitrogen was used as carrier gas at a flow rate of 2 mL min−1. Split injection was performed with a split ratio of 10:1 (v/v). The derivatives...
Capillary gas chromatographic (GC) determination of methylglyoxal (MGo) was developed on the basis of precolumn derivatization with 1,2-diaminopropane (DAP) at pH 3. The elution was carried out on an HP-5 (30m×0.32mm i.d.) connected with FID. The linear calibration curve was obtained for MGo within 0.09–1.04μg/ml with detection limit of 40ng/ml. Dimethylglyoxal (DMGo) also formed derivative with DAP...
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