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Developments in the field of tear film protein profiling are reviewed, including the advantages and disadvantages of the multiplex bead array technique and its potential for identifying biomarkers of ocular surface disease. Commercial kits, which specifically employ 96-well plates with polystyrene microspheres and magnetic beads, and sensitivity variations between them are discussed. Modifications...
Dry eye disease (DED) is a complex disorder of the ocular surface. Due to the lack of consistency between clinical signs and symptoms and poor clinical indicators of mechanisms and severity, there is a growing need for reliable biomarkers of the disease. The low volume of tears in DED is a source of technical challenges, although new technologies can now measure inflammatory cytokines or matrix metalloproteinases...
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