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PKCθ regulates the proliferation, survival and differentiation of T-cells. Here we show that PKCθ interacts with Raf-1 and B-Raf kinases. Raf-1 enhanced the kinase activity of associated PKCθ, while PKCθ reduced the catalytic activity of associated Raf-1. In contrast, B-Raf binding did not affect PKCθ kinase activity, and PKCθ did not change B-Raf activity. Coexpression of mutationally activated Raf-1...
An increase in 14-3-3 mRNA expression after hypoglossal nerve injury was demonstrated by RNA finger printing using the arbitrary primed polymerase chain reaction (RAP-PCR). RAP-PCR was carried out to compare differences in mRNA expression between axotomized (6 h after the transection) and normal hypoglossal nuclei in mice. The expression of several gene fragments was increased after nerve injury;...
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