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In this study, the SNPs located in the chicken pre-microRNAs were performed a genome-wide analysis. The result showed that pre-microRNA SNPs are negatively selected in respect to SNPs distribution between the pre-microRNA and their flanking regions. Interestingly, the seed regions show a higher SNP density than both the MIR and the other MIR regions excluding seed regions. Furthermore, the SNPs in...
This study aimed to study the immune activity of glutamate dehydrogenase (GDH) of streptococcus suis serotype 2 and detect its activity. METHODS: The GDH gene was cloned and sequenced. Recombinant GDH protein was expressed in a prokaryotic expression system and purified by affinity chromatography. Immunocompetenc of the resulting protein was analyzed by western blotting with anti-GDH monoclonal antibody...
The development of human cardiovascular systems physiology is inhibited by the lack of multiscale functional physiological data, which represents human heart physiology at the molecular, cellular, tissue, organ, and system levels. We have developed an experimental approach to study explanted human hearts in vitro at multiple physiological scales with a wide array of imaging modalities. This approach...
Idiosyncratic drug hepatotoxicity is a hepatotoxicity subset that occurs in a very small fraction of human patients, is poorly predicted by standard preclinical models and in clinical trials, and frequently leads to postapproval drug failure. Animal models utilizing bacterial LPS co-administration to induce an inflammatory background and hepatocyte cell culture models utilizing cytokine mix cotreatment...
There is a lot of plasma albumin in the lampetra japonica's buccal gland secretion. This protein has a unique and original position in the evolution of the albuminoid gene family[l]. This research does an analysis on the evolutional relationship between the plasma albumin of lampetra japonica and the other members of the albuminoid gene family by bioinformatics methods and made the evolution diagram...
Elucidation of the biological effects of the radio frequency (RF) electromagnetic fields has been investigated for decades but it is still not clear weather the observed effects are only due to hyperthermia or non-thermal effects exist as well. In this work, the effect of radio frequency (RF) radiation on protein expression in earthworms E. foetida using two-dimensional protein gel electrophoresis...
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