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Chronic imaging of the peripheral nervous system with contemporary techniques requires repetitive surgical procedures to reopen an area of interest in order to see underlying biological processes over time. The recurrence of surgical openings on an animal increases trauma, stress, and risk of infection. Such effects can greatly lessen the physiological relevance of any data recorded in this manner...
Our goal is to assess the feasibility of low-intensity ultrasound (US) combined with echocontrast agent microbubbles (MB) to transfect the ciliary muscle which has previously been demonstrated to potentially allow the secretion of therapeutic proteins. Reporter plasmid DNA encoding Gaussia luciferase (pCMV-Gluc-1), containing the LacZ gene (pVAX1-LacZ), alone or mixed with 50 % microbubbles (Artison),...
We present the detailed imaging of structures and processes of the nematode Caenorhabditis elegans (C. elegans) using non-linear microscopy (TPEF-SHG-THG). Consummative and specific information about the anatomy of the nematode was collected by implementing a combination of THG, SHG and TPEF image contrast modalities on the same microscope. Additionally, 3-D reconstruction of TPEF and THG images was...
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