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Methanolic extracts of different parts of Hyperium mysorense (HM) (Hypericaceae) namely aerial parts (HMA), flowering tops (HMF), leaf (HML), root (HMR) and stem (HMS) were screened for its in vitro and in vivo anti cancer activities. The short term toxicity studies were carried out against Ehrlich ascitic carcinoma (EAC) cells. Different concentrations of the HM extracts (1000 ??g/ml, 500 ??g/ml,...
To study the effect of Asparagus officinalis polysaccharide on the number and activity of erythrocyte complement receptor 1(CD35) in S180 mice. Red blood cells from mice venous blood are labeled by rat anti-mouse CD35 monoclonal antibody and FITC-conjugated goat anti-mouse antibody. Using flow cytometry, we determined the number of CR1. Using microscope, we studied the adherence between erythrocyte...
To study the effect of growth inhibition and induction of apoptosis in sarcoma 180 cells by juglone in vivo. S180 tumor-bearing mice were set up to investigate the effects of juglone on tumor growth inhibition and apoptosis. After HE staining, the pathomorphologic changes of tumor cells were observed by optical microscope. The ultrastructural changes of tumor cells were observed by transmission electron...
Chemotherapy is a common treatment for uveal melanoma. Rapid clearance of injected drug from the vitreous, necessitates repeated injections in to the eye. We have investigated the use of hydrogel particles to deliver chemotherapeutic drugs to the uveal melanoma site. Fluorescent dye-labeled nano- and micro-particles were injected intravenously into the animal. Particle accumulation in the uveal tissue...
Studies were conducted to establish the biochemical and pharmacological effects of W-2721 after irradiation in the mouse tests for up to 30 days of irradiation. Adult mice were irradiated with 3, 6, and 8 Gy of /sup 60/Co gamma rays with and without pretreatment by WR-2721 at a dose rate of 400 mg/kg body weight. Animals were sacrificed at different postirradiation days to estimate the amount of DNA,...
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