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Phosphorylation, the reversible addition of a phosphate group to amino acid side chains of proteins, is a fundamental regulator of protein activity, stability, and molecular interactions. Most cellular processes, such as inter‐ and intracellular signaling, protein synthesis, degradation, and apoptosis, rely on phosphorylation. This PTM is thus involved in many diseases, rendering localization and...
The demand for effective sample preparation strategies that enable the in-depth study of protein phosphorylation has spawned a number of selective affinity techniques that allow enrichment of phosphopeptides from highly complex peptide mixtures. Among those, metal oxide affinity (MOA) materials have seen a strong increase in popularity in recent years, and MOA chromatography is now one of the most...
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