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The effect of lipopolysaccharide (LPS) and turpentine on nitric oxide (NO) production were investigated in rats. Because of short half-life of NO in biological fluids, the plasma nitrite and nitrate concentrations (two stabile metabolites of NO) were measured based on Griess reaction, which is indirect assay for NO production. Injection of LPS at an intraperitoneal dose of 50μg/kg caused a 3,5-fold...
This study was designed to test the hypothesis that the activity and expression of iNOS in the ileum will be altered in young rats exposed to alcohol in utero (FAE). The subjects, 26-29-day-old rats, were progeny of dams fed an alcohol diet during gestation and their pair-fed/control cohorts. We determined the effects of endotoxin administration on ileal iNOS enzymatic activity and on ileal iNOS immunoreactivity...
Ca 2+ /calmodulin-dependent nitric oxide synthase was found to be induced during rat liver necrosis caused by administration of Propionibacterium acnes and E. coli lipopolysaccharide to rats. Examination of the specific induction of C a2+ /calmodulin-dependent NO synthase showed that the enzyme was induced in the lung, spleen and colon as well as the liver. Northern...
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