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A reversed-phase liquid chromatography coupled to tandem mass spectrometry (LC–MS/MS) method was developed and validated for simultaneous determination of ABT-888 and its major metabolite (M8) in human plasma. Sample preparation involved a liquid–liquid extraction by the addition of 0.25ml of plasma with 10μl of 1M NaOH and 1.0ml ethyl acetate containing 50ng/ml of the internal standard zileuton....
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