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In this study we have used rat hippocampal astrocytes in culture to investigate the effect of ethanol on kainate-induced glutamate secretion. Our results show that kainate (10μM to 500μM) stimulated glutamate release from astrocytes. Preincubation of astrocytes in the presence of ethanol induced a concentration-dependent (1mM–50mM) inhibition of glutamate release caused by stimulation of cells with...
We have employed rat hippocampal astrocytes in culture to investigate the effect of ethanol on reactive oxygen species (ROS) production as well as its effect on [Ca 2+ ] c and GFAP expression. Cells were loaded with the fluorescent probes fura-2 and H 2 DCFDA for the determination of changes in [Ca 2+ ] c and ROS production respectively, employing spectrofluorimetry...
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