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The seroprevalence of human granulocytic anaplasmosis (former human granulocytic ehrlichiosis, HGE) has been documented in several studies, but little data exists on incidence rates in healthy individuals. In a prospective study, we tested 125 healthy adults (mean age 43 years) - workers of the Białowieża Primeval Forest National Park, north-eastern Poland - for Anaplasma phagocytophilum IgG antibodies...
The aim of these investigations was to prepare the antigen and control sera for the ELISA. Positive control sera were obtained from 163 cows showing clinical symptoms of H. bovis infection and negative control sera came from 60 cows free from the infection. The antigen for the ELISA was prepared from H. bovis L1 larvae. Three basic fractions were isolated from whole excretory/secretory antigen by...
The prevalence of bovine viral diarrhea (BVD) virus infection on the basis of serological testing of cattle has been evaluated. Blood samples were analyzed in micro-seroneutralization test for the presence of BVD virus neutralizing antibodies against 1000 TCID₅₀ of NADL reference strain. Neutralizing antibodies were found in 83 per cent of animals tested. The percentage of seropositive animals was...
A new ELISA method for VHDV diagnosis (indirect sandwich ELISA) and Iiquid-phase blocking ELISA (LPBE) for the determination of antibodies to VHDV were developed. These assays are sensitive and specific and results may be directly correlated to those recorded by HA or HI. Besides, the presented techniques are rapid and relatively simple to perform and reagents are used economically. Therefore, the...
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