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The isolated H+-ATPase from Escherichia coli (EF0F1) was investigated by electron microscopy of samples of negatively stained monodisperse molecules, followed by single-particle image processing. The resulting three-dimensional maps showed that the F1-part is connected by a prominent stalk to a more peripheral part of F0. The F1-part showed stain-accessible cavities inside. In three-dimensional maps...
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