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Mitragyna speciosa (MS), a plant commonly known as kratom, is a widely used “legal high” opiate alternative for pain relief. DNA extracted from MS and 26 additional plant species was amplified by PCR using primers targeting the strictosidine beta‐D‐glucosidase (SGD) and secologanin synthase 2 (SLS2) genes and detected by high‐resolution melt curves using three intercalating dyes. Amplicon sizes were...
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