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In this study, culture supernatnats of Bacillus subtilis T-1 growing on brewery effluents and molasses was used for silver nanoparticles (Ag-NPs) synthesis. The biosurfactant production of B. subtilis T-1 was confirmed by the detection of genes in the genome and by the identification of the product in the supernatants. The genes for synthesis of surfactin (sfp, srfAA) and iturin (ituC) were noted...
To construct recombinant clone of glutathione S-transferase (GSTs) of Schistosoma japonicum (Sj) and express recombinant SjGSTs protein with his-tag that could be purified by single-step Ni2+-NTA affinity chromatography, one pair of primers was designed according to the sequence of GSTs gene. The DNA fragment of PGEX-KG GSTs gene was amplified by PCR with template PGEX-KG. Then it was cloned into...
Bifunctional alkylating agent 1,3-Bis-(2-chloroethyl)-1-nitrosourea (BCNU) is a significant anticancer drug in the clinical treatment of human malignancies. A serious complication of treatment with BCNU is the increased risk of a secondary leukaemia in long-term survivors because not all alkylating agent interactions with DNA result in cell death. In the present work, high-performance liquid chromatography...
This study aimed to study the immune activity of glutamate dehydrogenase (GDH) of streptococcus suis serotype 2 and detect its activity. METHODS: The GDH gene was cloned and sequenced. Recombinant GDH protein was expressed in a prokaryotic expression system and purified by affinity chromatography. Immunocompetenc of the resulting protein was analyzed by western blotting with anti-GDH monoclonal antibody...
An integrated fluidic system has been fabricated, capable of separating a mixture of different bio-molecules into its components. It is composed of a filter and an actuator; the pressure generated by the actuator sustains the flow of the mixture through the filter. The actuator is made by stacking several layers of conductive polymer. Actuator strain in excess of 10% has been obtained, which corresponds...
Ebp1 is a member of the PA2G4 family protein and initially isolated as an ErB3 (an epidermal receptor tyrosine kinase) binding protein. Ebp1 inhibits cell growth and repress transcription of E2F-regulated cell cycle genes. Previously, we reported that Ebp1 protein interacted with RNA polymerase subunit PB1 of the influenza virus and disturbed in vitro RNA synthesis by influenza RNA polymerase. Recently...
The objective of the study was to acquire the bovine enterokinase light chain (EKL) expressed in Pichia pastoris, which would be used in the cleavage and purification of fusion proteins. The fragment of EKL cDNA was obtained by RT-PCR from a sold bovine's duodenal mucosa, then cloned into the pUCm-T cloning vector and sequenced. Compared with the sequence deposited in GenBank, the cloned gene sequence...
In this paper, Southern blot analysis of the transgenic birch (Betula platyphylla Suk.) plants indicated that the the copy number of bgt varies from one to four. Of which 18.2 percent were single copy. Transcriptional expression of bgt gene in transgenic birch plants was analyzed by Northern blot. TGS has been found in four transgenic lines with one, three and four copies of bgt. Bgt gene was inserted...
Bifunctional alkylating agent 1,3-Bis-(2-chloroethyl)-1-nitrosourea (BCNU) is a significant anticancer drug in the clinical treatment of human malignancies. Several lines of evidences indicated that the cytotoxic activity of BCNU was related to their ability of inducing DNA interstrand cross-links (ICLs). In the present work, the formation of the ICLs from the treatment of calf thymus DNA with BCNU...
Hirudin has been well-characterized to be the strongest thrombin inhibitor with no affinity for other peptidases. Hirudin from natural sources is inefficient due to its low abundance in medicinal leech (Hirudo medicinalis). Though recombinant hirudin has been made by many laboratories, technical difficulties have limited recombinant hirudin from being used clinically. One of the difficulties is the...
Lycopene, a member of the carotenoids pigment family, is common in humans and found in the blood and tissues. Carotenoids include at least 600 pigments, most of which provide bright colors to various plants. Lycopene gives tomatoes and other fruits and vegetables a red color, while serving primarily to protect cells from photosensitization and to aid in photosynthesis. Lycopene is found in high concentrations...
Major obstacle in the management of fungal diseases is the availability of rapid diagnostic procedure. The growth-based phenotypic testing is still the method of choice in many microbiology laboratories. An ion-paired reverse phase chromatography (IP-RP)-based molecular assay has been developed. This molecular approach includes PCR amplification of ribosomal DNA followed by restriction fragment length...
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