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With the advent of new technologies, human exposure to ionizing radiation has increased. Therefore, appropriate pharmacological interventions and modalities are needed to protect humans against the deleterious effects of ionizing radiation. In this work, we developed a photosensitive, high-throughput chip-based assay for exploring the antioxidant [superoxide dismutase (SOD)]/radioprotective potential...
A TuMV-NIb gene fragment was amplified from the total RNA of Mustard leaves infected with TuMV-GZ strain by RT-PCR (Reverse Transcription Polymerase Chain Reaction). This fragment was cloned into plant binary vector pBI121 in the antisense orientation and transformed into Mustard (Brassica juncea L. Linshicaoyaozi) by Agro bacterium tumefaciens mediated method. 11transgenic T0 plants were obtained...
Understanding the genetic factors that promote recombinant protein accumulation in transgenic plants will provide insightful strategies for protein biofactory efficiency. Through combining biological and bioinformatics analysis, our work is to determine genetic and biological factors affecting increased protein accumulation of a bacterial cellulase enzyme in transgenic maize. Microarray experiments...
Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) is the key enzyme involved in CO2 fixation. It is an important enzyme for biomass production, especially for alternate energy production. Rubisco is an inefficient catalyst. Its carboxylase activity is compromised by numerous side-reactions including oxygenation of the sugar phosphate substrate by atmospheric O2. However, naturally occurring...
To study the effect of saponins of asparagus on apoptosis and the variations of Caspase-8, 9, 3 activity in the process of asparagus induced apoptosis in HepG-2. Cell was administrated with different concentrations of asparagus at different time, the apoptosis rate was determined by FCM with AV-PI staining, the morphology was observed by electron microscopy, Caspase-8, 9, 3 activities was measured...
Effects of NaCl stress on seed germination and seedling growth in wheat were studied in this paper. The results showed that the germination rate of the seeds treated with NaCl concentration at <;100 mM, germination rate of the seeds was not significantly comparing with control, however the germination of those seeds treated with NaCl at higher concentration (≥200 mM) was inhibited and at 400 mM...
The relationships among chlorophyll fluorescence parameters, chloroplast antioxidant enzyme and active oxygen in the leaves of P. tenuiflora seedlings under Na2CO3 stress was investigated in the article. Results showed that when Na2CO3 stress was less than 0.4%, the activity of antioxidant enzymes (APX, GST, SOD) increased along with the increasing Na2CO3 stress. However, the changes of actual photochemical...
Pectate lyase obtained the wide-spread application in the Chinese traditional medicine extraction because it can remarkably reduce impurity content and viscosity of the plant materials. However the low enzyme activity and monoicous species hindered the application of enzymatic method to the extraction process of the herbal medicine. In this paper, we constructed a engineering strain which can overexpressed...
Previous reports showed that the root extracts of Stellera Chamaejasme L had acaricidal activity against Tetranychus vienneusis Zacher. However, the acaricidal mechanism of the root extracts from Stellera Chamaejasme L against Tetranychus vienneusisis not clear. Here, the effects of the root extracts from Stellera Chamaejasme L. on the activities of three enzymes of mites: protease, glutathiona s-transferase...
In order to reveal effect mechanism of crop reactive oxygen species (ROS) metabolic system under exogenous salicylic acid (SA) to ozone (O3) stress, open top chambers were utilized to investigate the change of ROS production rate, lipid peroxidation extent, anti-oxidative enzymes activities and antioxidant content in soybean (Glycine max.) leaves. Exogenous SA treatment relieves the aggravation of...
β-ketoacyl-ACP synthetase II (KAS II) which catalyzes the elongation of 16:0-ACP to 18:0-ACP, determines the ratio of palmitic acid (16:0) to stearic acid (18:0). Using Rapid Amplification of cDNA Ends (RACE) and a peanut cDNA library we had previously constructed, we isolated a 1867bp cDNA of the KAS II gene containing a 1212bp complete open reading fragment (ORF). Sequence analysis revealed that...
Kosteletzkya virginica is a halophyte species which could be used for production of industrial raw material such as biodiesel and for developing ecologically sound saline agriculture as well as for tideland soil improving. Using Rapid Amplification of cDNA Ends (RACE) method, a 1610 bp cDNA of stearoyl-ACP desaturase (SAD) gene containing a 1188 bp complete open reading fragment (ORF) was firstly...
To understand the physiological and biochemical response of the lead-accumulator plant pseudostellaria maximowicziana (Franch.et Savat.) Pax to lead and zinc stresses, the pot experiment was used to study the protective enzymes (SOD, CAT and POD) activity and MDA content of pseudostellaria maximowicziana under lead and zinc stresses. The results showed as follows: with the increase of lead concentration,...
Acetyl-CoA carboxylase (ACCase; EC 6.4.1.2) catalyzes the synthesis of malonyl-CoA, the first intermediate in fatty acid synthesis. Genes for multi-functional acetyl-CoA carboxylase and two subunits of multi-subunit acetyl-CoA carboxylase, biotin carboxylase and β-Carboxyltransferase, have been cloned from peanut. The ORF of the three proteins are 6783bp, 1623bp, 1479 bp in length, encoding 2260,...
Dry seeds, water-soaked seeds and seedlings of rice were exposed to carbon ion radiation at doses of 0.02-20 Gy. Biological effects on survival, chromosomal aberrations and activity of protective enzymes against activated oxygen including superoxide dismutase (SOD) and catalase (CAT) as well as content of malondialdehyde (MDA) were detected. Oxidative damages were observed in both seeds and seedlings...
Increasing reach on natural antioxidants in foods and development of new assays have prompted critical reflection on the field. There are lots of plants have antioxidant activity in natural. Anthocyanins are phenolic compounds widely distributed in fruits and vegetables and their consumption has been shown to prevent some chronic diseases. They possess antioxidant activities and could be involved...
Betacyanins from Portulaca oleracea markedly reversed the D-gal induced learning and memory impairment by behavioral tests. The activities of SOD, CAT, GPx and GR in D-galactose-treated mice were enhanced. Significant negative correlations were found between the latency to find the platform of mice and the activities of SOD, CAT GR and GPx in mice brain. These results suggest the neuroprotective effect...
Retrotransposons are mobile genetic elements which transpose via an RNA intermediate. Because the transposition of retrotransposons causes insertional mutagenesis in a genome, the activation of retrotransposons has been used for basic and applied genome studies in plant biology. Some retrotransposons were shown to be transcriptionally activated by biotic and abiotic stress factors, such as UV light,...
ZmPP2C was a protein phosphatase 2C gene that we cloned from maize roots. It is an important type of protein phosphatase family. AlignX is a software which can compare homology of many proteins or nucleic acids and search homologous fragments or different bases in high similarity sequences. This paper makes use of the AlignX program to visualize and analyze DNA sequences of ZmPP2C comparing with other...
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