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A TuMV-NIb gene fragment was amplified from the total RNA of Mustard leaves infected with TuMV-GZ strain by RT-PCR (Reverse Transcription Polymerase Chain Reaction). This fragment was cloned into plant binary vector pBI121 in the antisense orientation and transformed into Mustard (Brassica juncea L. Linshicaoyaozi) by Agro bacterium tumefaciens mediated method. 11transgenic T0 plants were obtained...
Ceratoides lanata is the native sand-fixing plant in deserts of America Continent. As an even-green undershrub with extremely strong antifreeze capability, Ceratoides lanata had been considered as the winterfat and introduced into many countries including China. To explore the mechanism of cold resistance and to clone genes directly involving the strong antifreeze capability, a normalized cold-stressed...
Regulatory non-coding RNAs (ncRNAs) including miRNAs, play important roles in diverse biological processes including chromatin modification, mRNA synthesis or stability, and protein synthesis or repression. Now more and more experimental validated ncRNA have been founded, in fact there are more regulatory ncRNA than we expected, with increasing transcribed data, it is the time for us to data mining...
The first sequence obtained from a Potyvirus infecting Canna plants (Canna spp.) revealed that it is a new strain of Bean yellow mosaic virus (BYMV), without noticeable recombination with other plant RNA viruses from this region being found. Using a degenerate primer for all Potyvirus, a DNA fragment of about 1.6kb size was amplified from total RNA samples extracted from natural-diseased Canna plants...
Studies about the variation of DNA methylation pattern under high temperature stress (37°C) contribute to a comprehensive understanding of the apparent regulation of biological functions. Based on the in vitro cultivation of genetically modified birch (Betula platyphylla Suk.) clones for high-temperature stress, the paper detected exogenous gene transcription level differences with RT-PCR. Methylation-sensitive...
The transformation of cDNA into plant is still not carried out directly by a universal transgenic vector, hence it is not convenient for the transgenic application of those function-important cDNAs. Herein we introduced a rapid, cost-effective PCR-based DNA synthesis method to construct a new universal plant overexpression vector for the direct inserting of target cDNA during transgenic application...
cDNA containing the 3' part sequences of seven Lily symptomless virus (LSV) isolates were obtained by RT-PCR and sequenced. The sequenced region is corresponding to partial CP gene and the entire sequences of 16kDa gene located at the 3' end of LSV genome. The sequence homologies of partial CP and 16 kDa protein genes, compared with other reported isolates, were 87.4% to 100% and 97.9% to 100% respectively...
Pear is a commercially important fruit tree widely distributed in China that displays GSI (gametophytic self-incompatibility). It is necessary to determine S-alleles and S-genotypes for clarifying the compatibility between cultivars prior to pear planting and breeding. The S-genotype analysis using conventional method of artificial pollination is time consuming and requires much labor. In addition,...
Retrotransposons are mobile genetic elements which transpose via an RNA intermediate. Because the transposition of retrotransposons causes insertional mutagenesis in a genome, the activation of retrotransposons has been used for basic and applied genome studies in plant biology. Some retrotransposons were shown to be transcriptionally activated by biotic and abiotic stress factors, such as UV light,...
MicroRNAs (miRNAs) reported previously from Arabidopsis. thaliana and other seven plant species, by both experimental validation and prediction, were used to identify potential miRNAs in Saccharum officinarum which is an important food and bio-energy crop. A microfluidic microarray was developed for detecting plant miRNAs and used for the first experimental analyzing the mature miRNAs and miRNAs*...
The specific segment in 3' terminal region of strawberry mild yellow edge virus (SMYEV) genome was amplified by 3' rapid amplification of cDNA ends (RACE). Ten Chinese isolates were sequenced. Sequence analysis of coat protein gene showed nucleotide and deduced amino acid identities ranged from 76.8 to 100% and 86.4 to 100%, respectively. Phylogenetic analysis showed that all isolates of SMYEV fell...
The full-length cDNA of a new S-RNase (S34-RNase) (GenBank accession No.DQ414813) was successfully cloned from pistils of Chinese pear by RT-PCR and RACE methods. The S34-RNase shows a complete CDS of 687 nucleotides for putative 229 amino acids. The theoretical three-dimension structure of S34-RNase was predicted by homology modeling method and its structural characteristic was analyzed. Aligning...
Substantial differences occur between the male and female gametophytes of Laminariajaponica Aresch in morphology and physiology. The differences were investigated by screening a cDNA library generated by suppression subtractive hybridization (SSH). Using bioinformatics and northern blot analysis, we confirmed that two genes, Ihcf6 and fcp, were preferentially expressed in male gametophytes. Analyses...
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