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Forensic DNA analysis continues to be hampered by the complex interactions between metals and DNA. Metal ions may cause direct DNA damage, inhibit DNA extraction and polymerase chain reaction (PCR) amplification or both. This study evaluated the impact of metal ions on DNA extraction, quantitation, and short tandem repeat profiling using cell‐free and cellular (saliva) DNA. Of the 11 metals assessed,...
Metals can pose challenges while conducting forensic DNA analysis. The presence of metal ions in evidence‐related DNA extracts can degrade DNA or inhibit PCR as applied to DNA quantification (real‐time PCR or qPCR) and/or STR amplification, leading to low success in STR profiling. Different metal ions were spiked into 0.2 and 0.5 ng of human genomic DNA in an “inhibition study” and the impact was...