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Glutamate decarboxylase (GAD) is the key enzyme for the production of gamma-aminobutyric acid (GABA), a multifunctional agent which biosynthesis has attracted intense interest. Though immobilized GAD favors the preparation of GABA in several ways, its efficiency and enzyme activity recovery are damaged by the separation of purification and immobilization of enzyme. In order to simplified the process...
Intervertebral disc (IVD) degeneration is accompanied by a loss of proteoglycans (PGs) in the nucleus pulposus (NP), resulting in a loss of charged chondroitin sulfate (CS) chains, decreasing mechanical function of the disc. PG mimics may be employed to restore NP functionality. Synthesis of CS bottle brush structures requires immobilization of CS at its terminal end. In this study, we have investigated...
This paper presents a novel Ni-Co alloy coated QCM biosensor fabricated by electroplating nickel and cobalt on the surface of 10-MHz AT-cut QCM. The biotin was immobilized on the coated surface by the affinity mechanism of Ni-Co alloy. Then, the immunoreactions can be performed on the QCM biosensors. The results show that the Ni-Co alloy coated QCM biosensor can provide specific binding with His-tagged...
Bovine serum albumin (BSA) was space selectively immobilized onto a quartz substrate using photo-reactions initiated by pulsed laser irradiation of benzophenone derivatives tethered by silane coupling to the quartz. The excitation of the benzophenone with 266 nm light yields the pi-pi* triplet state with a quantum yield of 1. This triplet state then reacts with BSA in buffer solution in contact with...
Immobilization of bacteriophage T4 onto the SPEs was achieved by formation of amide bonds between the protein coating of the phage and the electrochemically generated carboxylic groups at the carbon surface. The immobilized T4 were then used to specifically detect target bacteria (E.coli). Impedance measurements (Nyquist plots) show significant shifts in both the real (Zr) and imaginary (Zi) impedances...
The direct electron transfer between electrodes and glucose oxidase (GOD) immobilized in a matrix containing zirconium dioxide nanoparticles (ZrO2) is described. The protein-nanoparticle assembly is stabilized by charged and uncharged compounds and the direct electron transfer is enhanced. The effects of different compositions on the electrochemical parameters, formal potential, surface loading, and...
A novel optical biosensor technique is being investigated for early detection of myocardial infarction utilizing the distance-dependent chemical transduction method of fluorescence resonance energy transfer (FRET). The FRET process requires two fluorophores termed the donor and the acceptor. When in close proximity, the donor absorbs energy from the excitation source and nonradiatively transfers the...
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