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We report, for the first time, the use of single poly (methyl-methacrylate) (PMMA) polymer-based nanopores to not only detect the translocating biomolecules of dsDNA and BSA in individual populations, but also distinguish between them in a mixed population, based on the obtained depths (\(i_{\mathrm {\mathbf {Block}}}\) ) and durations (\(t_{D}\) ) of the ionic current blockade events. Quantitative...
Poly(glycerol sebacate) (PGS) has become one of the most promising new tissue engineering scaffold materials-of-construction. We hypothesized that the lipase inhibitor Orlistat might be suitable for inhibiting lipase-mediated PGS degradation. Here we investigated whether Orlistat can: (1) be physically transported into PGS using 70% (v/v) ethanol in water as a carrier, (2) be stably entrapped within...
Nano-indentation studies using atomic force microscopy (AFM) were introduced to investigate the effects of hydrolysis degradation on mechanical properties of polyglactin 910 monofilament sutures. The polyglactin 910 sutures were immersed in phosphate buffered saline (PBS) solution of pH 5, 7.4, 10 without enzyme and pH 7.4 with esterase enzyme. After that, the samples were incubated at 37°C under...
Conducting polymer microstructures for enzymatic biosensors are developed by a facile electrochemical route. Horse radish peroxide entrapped polypyrrole films with bowl shaped microstructures are developed on stainless steel (SS 304) substrates by a single step process. Potentiodynamic scanning/cyclic voltammetry is used for generation of polypyrrole microstructures using electro generated oxygen...
This paper reports a novel nanofabrication method for living cell. Our method provided both nano deposition and ablation on a living cell in nanoscale resolution. This paper shows nanostructures fabrication using this electron beam (EB) induced deposition, and also reports cell response for EB direct drawing. Conventional onsite processes for the live cells were reported as femto second laser induced...
The purpose of this research was to develop a sustained micosphere for recombinant human granulocyte colony-stimulating factor (RHG-CSF). RHG-CSF was first incorporated into glassy sodium hyaluronate (HA) particle according to the freezing-induced phase separation method previously reported by the authors' group. Once G-CSF was incorporated in the glassy HA particles, it gained resistance to organic...
Modified polyaspartic acid (PASP-SEA-ASP) was prepared from polysuccinimide (PSI) that was thermal condensation polymer of aspartic acid (ASP), taurine (SEA) and ASP. The property of scale inhibition, dispersant and biodegradation of PASP-SEA-ASP were studied. The structure of modified polyaspartic acid was characterized by means of FTIR. Calcium carbonate crystals in scale samples were analysed by...
A novel antibacterial material (P-PET) was prepared by immobilizing ε-polylysine on polyethylene terephthalate (PET) nonwoven fabrics in this study. Surface modifications of the fabric were performed by chemically modified procedure: creating carboxyl groups onto PET surface, grafting coupling agent and immobilizing the ε-polylysine. Scanning electron microscopy (SEM) was used to analyze the surface...
In the present study we reported the feasibility of extracellular biopolymer(PF-9) produced by Pseudomonas fluorescence C-9 as an alternative biosorbent to remove Ni(II) metallic ions from environmental and industrial wastewater. The ability of PF-9 to remove the Ni(II) ions was investigated by using batch biosorption procedure. The effects such as pH, dosage of biosorbent, Ni(II) initial concentration...
Gold - poly(dimethylsiloxane) (Au - PDMS) nanocomposite freestanding films were prepared and used to study the binding of Human Serum Albumin (HSA) to polyclonal anti-HSA. A new method of synthesis was developed for sensing application when most of the gold nanoparticles have to be localized in the surface layer. The localized surface plasmon resonance band in gold nanoparticles is used to monitor...
Natural materials exhibit anisotropy with variations in soluble factors, cell distribution, and matrix properties. The ability to recreate the heterogeneity of the natural materials is a major challenge for investigating cell-material interactions and for developing biomimetic materials. Here we present a generic fluidic approach using convection and alternating flow to rapidly generate multi-centimeter...
Modification to surface texture of biomaterials, protein adsorption and interactions of biomaterials and cells have been studied in vitro for several decades in tissue engineering. However, few reports focus on the decoration of in vivo environment to implanted biomaterials and the effects of the decoration on cells proliferation and wound healing. In this study, we used porous silk fibroin materials...
We have utilized a cytoskeleton, microtubule (MT), as a template for conductive nanowires. Biotin-labeled MTs were coated with streptavidin-labeled nanogold (NG) particles. Labeling of two modified MTs, biotin-labeled and NG-coated MTs, were evaluated by fluorescent and scanning electron microscopy, which verified the specific binding of NG particles on biotin-labeled MTs. Then, NG-coated MTs were...
In this work, an array of quartz crystal microbalance (QCM) sensors is fabricated on a single quartz crystal substrate and attached with poly(dimethylsiloxane) (PDMS) micro chamber for using in flow-injection system. The Cr/Au electrode array is deposited on both side of quartz substrate by sputtering through electroplated microshadow mask. PDMS micro chamber was fabricated by mold casting technique...
An optical biosensor based on gold nanohole arrays was developed using a new nanosphere lithography technique. A vertical deposition method was used to deposit multilayers of a polystyrene (PS) - gold composite on a silanized glass substrate. The SEM images have shown that the gold nanohole and nanoring arrays are distributed uniformly on the substrate. The sensitivity of the gold surface plasmon...
Porous chitosan/collagen scaffolds were investigated via biological evaluation to learn whether it could be used for periodontal tissue engineering. Periodontal ligament cells (PDLCs) were co-cultultured with chitosan/collagen scaffold and cytocompatibility were evaluated by MTT assay, as well as SEM and CLSM observation. PDLCs' mRNA expression of ECM was carried out by RT-PCR tests. For tissue compatibility...
Periodontal tissue engineering represents a possible approach to regenerate the human periodontal ligament (PDL) around dental implants. The aim of this study was to observe the morphological and biological property of the human periodontal ligament cells that were three-dimensional (3D) cultured onto PGA scaffolds in vitro. The human periodontal ligament cells were seeded and cultured onto PGA 3D...
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