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The embryonal carcinoma P19 cells provide a model to study neuronal differentiation. Cells that are exposed to retinoic acid become mature neurons within a few days with a pronounced axonal and dendritic polarity. Notably, an accelerated rate of neurite extension characterizes densely but not sparsely plated cells. DNA microarray experiments show maximal differences in gene expression of the dense...
Antisense oligonucleotides can selectively block disease-causing genes due to the specificity of the Watson–Crick base-pairing mechanism of action. A genome-wide view of antisense technology is illustrated via protein kinase A RIα antisense. Complementary DNA microarray analysis of the RIα antisense-induced expression profile shows the up- and down-regulation of clusters of coordinately expressed...
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