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In this work we present a hyperspectral imaging device (HSI) developed at INRIM and integrated in an inverted microscope for application in multi labeled fluorescence microscopy. The HSI device is realized with a scanning Fabry-Perot interferometer and a fast CMOS camera integrated in an optical microscope. The HSI camera alone has been tested for some fluorescent molecules in multi-well samples to...
This study presents a portable noninvasive detection system which can get the image of specific spectral immediately, that promote to oral cancer diagnosis. According to research literature show the performance called autofluorescence of cells after exciting by specific wave band of light. It is different of the spectrum distribution for lesions of epithelial cells and normal cells after fluorescence...
The research team is focused on the development of a chip-sized spectroscopy microsystem for the detection of gastrointestinal (GI) early cancerous lesions. The paper will briefly cover the outstanding properties of diffuse reflectance and fluorescence spectroscopy detection and the concept of cointegration of highly selective thin-films optical interference filters on silicon photodiodes, in order...
We present the results of a study in which we monitored anti-angiogenic therapy response in a mouse cancer model with in-vivo dynamic fluorescence imaging. In the experiments indocyanine green (ICG) was used as a fluorescent optical contrast agent and its time-dependent uptake and washout before and after the administration of bevacizumab was recorded. We found that compared with other organs, tumors...
This paper describes the design of a miniature, cost-effective spectroscopy system for assessing tissue biochemical and morphological information using a few wavelengths. This instrument will integrate thin-film optical filters and silicon photodiodes, avoiding the use of a spectrograph and optical fibers. The components in the set-up design are described. The feasibility of using only 16 wavelengths...
Human tissues show autofluorescence emission spectra when excited by ultraviolet or short-wavelength visible light. The intensity and shape of these spectra depends of the tissues pathologic state. Therefore, its measurement can give information about the degree of malignant transformations that could lead to cancer. This paper describes the design of an integrated CMOS compatible fluorescence measurement...
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