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W pracy zostały opisane biosensory proteinowe oraz ich właściwości fluorescencyjne. Przybliżono podstawy projektowania biosensorów proteinowych oraz strategii stosowanych przy projektowaniu sensorów białkowych:rational protein design (racjonalne projektowanie), directed evolution (ewolucja ukierunkowana), de novo design (projektowanie białek de novo).Zestawiono anality oraz proteiny receptorowe wykorzystywane...
The green fluorescent protein (GFP) from the jellyfish Aequorea victoria has been extensively used as a marker of gene expression and protein targeting in intact cells and organisms. Since GFP has no toxic effect on cells and it is easy to detect, it can replace controversial transformation markers such as those for antibiotic or herbicide resistance. The protein can also be used to detect dynamic...
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