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In this study, culture supernatnats of Bacillus subtilis T-1 growing on brewery effluents and molasses was used for silver nanoparticles (Ag-NPs) synthesis. The biosurfactant production of B. subtilis T-1 was confirmed by the detection of genes in the genome and by the identification of the product in the supernatants. The genes for synthesis of surfactin (sfp, srfAA) and iturin (ituC) were noted...
Radio Frequency Identification (RFID) is a very promising wireless technology able to trace and track individual objects. The pharmaceutical supply chain is a challenging scenario, where an item-level traceability is crucial to guarantee transparency and safety in the drug flow. Unfortunately, there are still some barriers limiting the large-scale deployment of these innovative technologies. In order...
Recently, liquid chromatography coupled to mass spectrometry (LC-MS) has become a standard technique for identifying differential abundance of peaks as biomarkers. Two major problems in the preprocessing of LC-MS data analysis are how to adjust and align multiple LC-MS datasets efficiently and correctly. Hence, an effective algorithm is needed to adjust the variation in retention time and align protein...
To construct recombinant clone of glutathione S-transferase (GSTs) of Schistosoma japonicum (Sj) and express recombinant SjGSTs protein with his-tag that could be purified by single-step Ni2+-NTA affinity chromatography, one pair of primers was designed according to the sequence of GSTs gene. The DNA fragment of PGEX-KG GSTs gene was amplified by PCR with template PGEX-KG. Then it was cloned into...
The Radio Frequency Identification (RFID) is a wireless technology that is becoming more and more important as auto-identification solution for many application scenarios. The adoption of this innovative technology in the pharmaceutical sector promises to solve several problems related to tracing and tracking systems at item level. Unfortunately, there are still some barriers limiting the largescale...
The item-level traceability is a very important requirement for many practical application scenarios, where it needs to guarantee perfect transparency for products flow along the whole supply chain. Among these, the pharmaceutical distribution is a very interesting scenario, characterized by many challenges, where, the Radio Frequency Identification (RFID) technology will play a very important role...
By improving chromatographic resolution and Mass Spectrometry scanning method, an isotope dilution method which can detect Naphthalene, Acenaphthene, Phenanthrene and Chrysene in water samples is established. Compared with Liquid-liquid Extraction, double Purification of solid phase extraction column and Silica Gel Column is more suitable for large volume injection. Their retention behavior of solid...
Non-mitogenic form of recombinant Human acidic fibroblast growth factor (nrhaFGF) retained the cardio and neuro-protective characters of the wild-type aFGF while decreased the potential side effects like mitogenesis activity. In this paper, nrhaFGF was successfully expressed in Escherichia coli and yielded about 5% of the total soluble protein in the 10L fermentation tank. the succeedent chromatography,...
Gas chromatography-mass spectrometry (GC-MS) was used to study the compounds from ether extract of Catharsius molossus L., the traditional Chinese medicine, 'dung beetle'. Over twenty kinds of compounds from the ether extract were detected, and fifteen compounds whose match index was more than 800 were identified. All the identified compounds belonged to fatty acids, and the extract was rich in unsaturated...
A novel method to determine semi-volatile organic compound(semi-VOC) was established by HPLC coupled with a partly-immersed three-phase hollow fiber liquid phase microextraction (PI-LPME) technique for sample preparation. The influence of extraction parameters including organic solvent, pH of donor and acceptor phase, extraction time, the proportion of hollow fiber in gas and liquid were optimized...
The aim of the study was to develop a procedure of purifying rabbit anti-rat TRβΔ polyclonal antibody from the antiserum. TRβΔ was a newly reported thyroid hormone receptor beta isoform. To gain better understanding of this new receptor, such as its tissue distribution, its specific antibody was needed. In this study, chemically synthesized peptide, which is uniquely existed in the TRβΔ, was used...
The new chemical method for identifying and quantifying Sudan I (1-[(2,4-dimethylphenyl)azo]-2-naphthalenol) from chilli species is devised to overcome the shortcoming with complex operation, longer analytical period of High performance liquid chromatography (HPLC) assay. Sudan I complexed with Cu2+ leads to the shift of visible absorption wavelength from 478 nm to 526 nm. The major product is the...
A salting-out phase separation microextraction /liquid chromatography method was used for the simultaneous determination of three phenols in water, including α-naphthol, β-naphthol and bisphenol A. The chromatographic conditions: Diamonsil C18 column (5μm, 150×4.6 mm i.d.) was used; The column temperature was room temperature; The mobile phase was methanol / water (57:43); The flow rate was 1.0 mL/min;...
This study aimed to study the immune activity of glutamate dehydrogenase (GDH) of streptococcus suis serotype 2 and detect its activity. METHODS: The GDH gene was cloned and sequenced. Recombinant GDH protein was expressed in a prokaryotic expression system and purified by affinity chromatography. Immunocompetenc of the resulting protein was analyzed by western blotting with anti-GDH monoclonal antibody...
Metabonomics approach based on the GC-MS technique was used to characterize the perturbation of volatile and semi-volatile components in zebrafish embryos induced by bisphenol A (BPA) for 8 d since fertilization. The exposure concentrations of BPA were 0, 0.5, 1.5 and 4.5 mg/L, around assumed a "safe level" (1.5 mg/L) that stated by US EPA. Results indicated that, the relative proportions...
Sample preparation is essential for isolating desired components from complex matrices and greatly influences their reliable and accurate analysis. Stir-bar-sorptive extraction (SBSE) is a new and effective sample preparation technique.A rapid method for analysis of Ultra-trace nonylphenol in the aquatic environment was developed by stir bar sorptive extraction. The extraction recoveries of nonylphenol...
A newly isolated moderate thermophilic strain 1-7 producing alkaline and thermostable lipase previously was identified as Acinetobacter calcoaceticus, which was isolated from the soil collected from bay of Bohai in China. The lipase showed maximum activity at pH 9.0 and high thermostability. The lipase from Acinetobacter calcoaceticus 1-7 was purified by ammonium sulfate precipitation, ion exchange...
Various extracts of Wikstroemia chamaedaphne were evaluated their acaricidal activities against Tetranychus cinnabarinus (Boisduval) and Tetranychus urticae Koch (Acari: Tetranychidae) in laboratory. According to our study, the Wikstroemia chamaedaphne extracts had significant acaricidal activities against T. cinnabarinus and T. urticae. In this study, the mean LC50 value for T. cinnabarinus and T...
Alkaline phosphatase was isolated and purified from the cattle (Bos taurus ) liver by means of homogenizeation, n-butanol disposal, thermal denaturation, ultrafiltration, DEAE- Sepharose column chromatography and Sephacryl S-200 gel filtration chromatography. Its specific activity was 19863.50, and the purification fold was 228.74. Its optimum pH and temperature was 10.0 and 45°C, respectively. Na...
A series of specially designed agarose-based HIC adsorbents were examined using human immunoglobulin G (IgG) and human serum albumin (HSA) as model proteins, to investigate the effects of structural properties of both proteins and stationary phases on the adsorption behavior in HIC. The adsorbents examined differ from each other in terms of ligand density, spacer arm and ligand type. Dynamic binding...
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