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Analysis of blood biochemical parameters is an important aspect of toxicological studies in fish. Anesthetic is typically used before blood sampling to minimize injury to the fish; however, anesthetic may also act as a stressor and alter biochemical measurements. To better understand blood responses to anesthetic stress, we evaluated biochemical alterations in the blood serum of endangered Chinese...
To determine whether modulating reactive oxygen species (ROS) release by mitochondrial is involved in the cardioprotection exerted by acetylcholine (ACh). In isolated ventricular myocytes from male Sprague-Dawley rats, 0.1 ??M ACh was administered for 6 min before 60 min of simulated ischemia and 30 min of reoxygenation or reperfusion (I/R). A mitochondrial ATP-sensitive potassium channel (mitoKATP...
In this study, the 5' terminus of ldhL gene in Lactobacillus casei ATCC 334 was fused to the 3' terminus of pdc gene promoter (Ppdc) part in Zymomonas mobilis ATCC 10988 by recombinant PCR. The fusion gene Ppdc-ldhL was integrated in broad-host-range plasmid pBBR1MCS3, transformed by electroporation and expressed in Z. mobilis ATCC 10988. The primary expression of heterologous lactate dehydrogenase...
With development of nanotechnology application, industry and government regulators notice that nanomaterials, especially nanoparticles, have some potential intimidation for human health and environmental safety. In this work, the cytotoxicity of 20 nm and 200 nm polystyrene nanospheres was investigated in cultured L929 fibroblast cells. There was an obvious difference of cytotoxicity between 20 and...
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