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We examine the use of an ensemble method, Random Forests, to delimit subspecies using mitochondrial DNA (mtDNA) sequences. Diagnosability, a measure of the ability to correctly determine the taxon of a specimen of unknown origin, has historically been used to delimit subspecies, but few studies have explored how to estimate it from DNA sequences. Using simulated and empirical data sets, we demonstrate...
The marsupials of the family Didelphidae went through profound taxonomic rearrangements in recent decades, mainly related to an increase in the number of specimens deposited in scientific collections and the inclusion of molecular data in systematic analyses, resulting in better resolved phylogenies and taxa delimitation. Analyses of a large series of the gracile mouse opossum Gracilinanus agilis,...
Sequence variation of a 1066 bp long mtDNA fragment (cytochrome b gene, adjacent part of tRNA-Thr gene) of four known-locality samples of Testudo kleinmanni (Tripolitania, Libya) and of four samples of T. werneri (Negev, Israel) is compared with additional five sequences of pet trade tortoises allegedly representing T. kleinmanni. Four haplotypes, differing in one to four mutation steps occur. The...
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