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The PDC1 gene encoding a pyruvate decarboxylase in S. cerevisiae and Kanr gene were respectively amplified by PCR using oligonucleotides which contained the restriction of NotI, EcoRI, and BglII. The two genes were digested with the same EcoRI and BglII, and linked together for inserting the Kanr gene into the PDC1 gene while the gene disruption cassette was constructed. After transformation of the...
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