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A novel platform based on gold nanoparticles (Au NPs) coupled with double-stranded DNA sequences and aptamers (referred to as ds(sgc8c)-Au NPs) was developed for ultrasound-controlled targeted drug delivery. The sgc8c aptamers with high affinity to CCRF-CEM (T-cell leukemia) cells facilitated the cellular uptake of ds(sgc8c)-Au NPs. The aptamers were extended with repeated CG base pairs for loading...
The detection of Hg2+ ions at the nanomolar level was achieved using a DNA aptamer as the molecular recognition element. The single stranded DNA sequence known as thrombin binding aptamer (TBA) was conjugated to a quantum dot and a gold nanoparticle to create the mercury-sensing probe. The quantum dot donor and gold nanoparticle acceptor allowed for optical detection via energy transfer using a spectrophotometer...
In this work, the effect of gold nanoparticles (AuNPs) with diameters of around 5, 10 and 20 nm on PCR efficiency is evaluated respectively using a real-time PCR machine. Gold nanoparticles show no obvious effect on PCR at low particle concentration. When the concentration is increased, PCR inhibition is observed. At the same particle concentration, gold nanoparticles of different sizes show different...
We previously demonstrated the first DNA-nicking agent using PEG linked photoactive compound with gold nanoparticles (3 nm). The DNA cleavage was greatly improved (104 times) by nanoparticle conjugation compared to photoactive compound alone. However, the compound failed to recognize gene sequence and thus greatly limit their biomedical applications. In this study, we demonstrated the use of gold...
In recent years, quartz crystal microbalance (QCM) plays an important role in many biological detections, and the enhancement of a single strain DNA probe linked to the sensor surface is of crucial importance in DNA molecule recognition. By means of nanogold modification and mass amplification, a QCM biosensor for Escherichia coli O157:H7 DNA detection was explored and developed in this work. As a...
Different from traditional detection methods which were expensive and time consuming for biochemical analyses, a DNA chip assay by using gold nanoparticles (AuNPs) immunological amplification methods and radio frequency techniques was applied to detect DNA sequences rapidly and conveniently. First the capture DNA (cDNA) was immobilized to the substrate on which the LC shunt located. Then the target...
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