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The transition path is a tiny fraction of a molecular trajectory during which the free-energy barrier is crossed. It is a single-molecule property and contains all mechanistic information of folding processes of biomolecules such as proteins and nucleic acids. However, the transition path has been difficult to probe because it is short and rarely visited when transitions actually occur. Recent technical...
AFM-based nanomanipulation has been used to study every type of biological macromolecules and revealed important, previously unknown properties and functional mechanisms. The capacity of the AFM for the isolation, transfer, positioning and assembling of individual macromolecules with nanometer spatial resolutions has significantly advanced the field of bionanotechnology toward the fabrication of functional...
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