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Recently, there has been an increasing interest to propose computational approaches based on image processing to automate the comparison of spatial gene expression patterns contained in transgenic embryo images. The first step is typically to classify the images between lateral and dorsal/ventral views, and then to align them along the anterior and posterior ends. While some methodologies have been...
This paper presents an advanced diascopic illumination technique for simultaneously multi-wavelength fluorescence excitation and detection without any filter sets. High-throughput capillary electrophoresis detection can be realized with this simple and novel approach. The developed system is composed of a home-built dark-field condenser for fluorescence excitation and an ultraviolet-visible-near infrared...
We calculated the field pattern above hole-arrays with an analytical model that we developed. Spectral imaging measurements strongly confirm the model. Such a pattern can be used for high resolution fluorescence measurements of biological samples.
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